Review

igfbp3 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc igfbp3

AKT phosphorylation of the upstream kinase cascade of the Hippo pathway is negatively regulated by PKCη. a Western blot analysis showing the activation of members of the upstream kinase cascade of the Hippo pathway, which is regulated by AKT phosphorylation, in PKCη KO TNBC cells. PKCη promotes YAP and PTEN expression, leading to dephosphorylation and inactivation of AKT. b An AKT inhibitor (MK-2206) effectively inhibits the upstream Hippo pathway phospho-cascade regulated by AKT in PKCη KO TNBC cells. c YAP-specific siRNA downregulates PTEN expression in TNBC cells in conjunction with AKT activation. d Relative mRNA expression of PTEN and PRKCH in control and MDA-MB-231 PKCη KO cells, as determined by RT‒qPCR. Knockout of PKCη in MDA-MB-231 cells resulted in a marked reduction in PTEN transcript levels compared with those in control cells. e Impact of PKCη expression on YAP transcriptional targets in TNBC cells. f Relative mRNA expression of canonical YAP target genes ( AXL, CYR61, <t>IGFBP3</t> , and TEAD ) was measured via RT‒qPCR in MDA-MB-231 PKCη KO and control cells. PKCη knockout results in a significant reduction in the expression of these downstream effectors. g Schematic illustration of how YAP activity regulates PTEN expression and modulates the AKT pathway through this feedback mechanism (in the presence or absence of PKCη). Statistical significance was determined via two-way ANOVA, where * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Igfbp3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igfbp3/CYR61+XP+Rabbit+mAb/pmc12910040-414-66-67
Average 95 stars, based on 95 article reviews

igfbp3 - by Bioz Stars, 2026-10

95/100 stars

Images

1) Product Images from "PKC-eta promotes breast cancer metastasis by regulating the Hippo–YAP signaling pathway"

Article Title: PKC-eta promotes breast cancer metastasis by regulating the Hippo–YAP signaling pathway

Journal: Signal Transduction and Targeted Therapy

doi: 10.1038/s41392-026-02572-0

Figure Legend Snippet: AKT phosphorylation of the upstream kinase cascade of the Hippo pathway is negatively regulated by PKCη. a Western blot analysis showing the activation of members of the upstream kinase cascade of the Hippo pathway, which is regulated by AKT phosphorylation, in PKCη KO TNBC cells. PKCη promotes YAP and PTEN expression, leading to dephosphorylation and inactivation of AKT. b An AKT inhibitor (MK-2206) effectively inhibits the upstream Hippo pathway phospho-cascade regulated by AKT in PKCη KO TNBC cells. c YAP-specific siRNA downregulates PTEN expression in TNBC cells in conjunction with AKT activation. d Relative mRNA expression of PTEN and PRKCH in control and MDA-MB-231 PKCη KO cells, as determined by RT‒qPCR. Knockout of PKCη in MDA-MB-231 cells resulted in a marked reduction in PTEN transcript levels compared with those in control cells. e Impact of PKCη expression on YAP transcriptional targets in TNBC cells. f Relative mRNA expression of canonical YAP target genes ( AXL, CYR61, IGFBP3 , and TEAD ) was measured via RT‒qPCR in MDA-MB-231 PKCη KO and control cells. PKCη knockout results in a significant reduction in the expression of these downstream effectors. g Schematic illustration of how YAP activity regulates PTEN expression and modulates the AKT pathway through this feedback mechanism (in the presence or absence of PKCη). Statistical significance was determined via two-way ANOVA, where * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Techniques Used: Phospho-proteomics, Western Blot, Activation Assay, Expressing, De-Phosphorylation Assay, Control, Knock-Out, Activity Assay

Blocking Assay:

Article Title: Intrauterine botulinum toxin A administration promotes endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in thin endometrium
Article Snippet: In brief, uterus tissues were minced by a homogenizer, lysated with ice-cold RIPA buffer, and protein lysates were separated by SDS-PAGE and transferred onto PVDF membrane (Millipore; Billerica, MA.). .. After blocking in 5% BSA in 1X TBS buffer containing 0.1% Tween-20 for 1 h at RT, the membranes were incubated with primary antibodies against integrin β3 (Cell Signaling; #13166, 1:1000), OPN (Enzo; ADI-905–629, 1:1000), VEGF (Invitrogen; MA5-13182, 1:500), IGFBP3 (Novus; NBP2-12364, 1:500), and β-actin (Cell signaling; 3700S, 1:1000) for overnight, washed and reacted with HRP-conjugated mouse or rabbit secondary antibodies (BIORAD; 170–6516, 170–6515, 1:3000). .. Signals were developed using ECL solution (Thermo; 32106) and immunoreactive bands were visualized by LAS-4000.

Article Title: Intrauterine botulinum toxin A administration promotes endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in thin endometrium.
Article Snippet: In brief, uterus tissues were minced by a homogenizer, lysated with ice-cold RIPA buffer, and protein lysates were separated by SDS-PAGE and transferred onto PVDF membrane (Millipore; Billerica, MA.). .. After blocking in 5% BSA in 1X TBS buffer containing 0.1% Tween-20 for 1 h at RT, the membranes were incubated with primary antibodies against integrin β3 (Cell Signaling; #13166, 1:1000), OPN (Enzo; ADI-905–629, 1:1000), VEGF (Invitrogen; MA5-13182, 1:500), IGFBP3 (Novus; NBP2-12364, 1:500), and β-actin (Cell signaling; 3700S, 1:1000) for overnight, washed and reacted with HRP-conjugated mouse or rabbit secondary antibodies (BIORAD; 170–6516, 170–6515, 1:3000). .. Signals were developed using ECL solution (Thermo; 32106) and immunoreactive bands were visualized by LAS-4000.

Incubation:

Article Title: FTO‐mediated m 6 A demethylation regulates IGFBP3 expression and AKT activation through IMP3‐dependent P‐body re‐localisation in lung cancer
Article Snippet: .. The membranes were blocked with 5% skim milk in 37°C and then incubated overnight at 4°C with primary antibodies against human FTO (Proteintech, China, 27226‐1‐AP), IGFBP3 (CST, USA, 64143), AKT (CST, USA, 9272), and phosphorylated (p)‐AKT (S473) (CST, USA, 4060), p‐AKT (T308) (CST, USA, 13038), GAPDH (Abcam, UK, ab181602), β‐actin (Abcam, UK, ab8227), Mettl3 (Proteintech, China, 15073‐1AP), Mettl14 (Proteintech, China, 26158‐1AP), IMP3 (Proteintech, China, 14642‐1AP), IMP2 (Proteintech, China, 22803‐1AP), IMP1 (Proteintech, China, 11601‐1AP), YTHDF1 (Proteintech, China, 17474‐1AP), YTHDF2 (Proteintech, China, 24744‐1AP), YTHDF3 (Proteintech, China, 25537‐1AP), YTHDC1 (Proteintech, China, 14392‐1AP), PATL1 (Proteintech, China, 21631‐1AP), LSM14A (Proteintech, China, 18336‐1AP), Tubulin (Proteintech, China, 80762‐1RR), Histon H3 (Proteintech, China, 17168‐1AP), PCNA (CST, USA, 13110), Annexin V (CST, USA, 8555). .. After washing, the membranes were incubated with horseradish peroxidase (HRP)‐conjugated secondary antibodies (Abcam, UK, ab205718) at 37°C for 1 h. Protein bands were visualised using an enhanced chemiluminescence detection kit (Yamei, China) and quantified by densitometry using a Windows system with ImageJ software.

Article Title: Intrauterine botulinum toxin A administration promotes endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in thin endometrium.
Article Snippet: In brief, uterus tissues were minced by a homogenizer, lysated with ice-cold RIPA buffer, and protein lysates were separated by SDS-PAGE and transferred onto PVDF membrane (Millipore; Billerica, MA.). .. After blocking in 5% BSA in 1X TBS buffer containing 0.1% Tween-20 for 1 h at RT, the membranes were incubated with primary antibodies against integrin β3 (Cell Signaling; #13166, 1:1000), OPN (Enzo; ADI-905–629, 1:1000), VEGF (Invitrogen; MA5-13182, 1:500), IGFBP3 (Novus; NBP2-12364, 1:500), and β-actin (Cell signaling; 3700S, 1:1000) for overnight, washed and reacted with HRP-conjugated mouse or rabbit secondary antibodies (BIORAD; 170–6516, 170–6515, 1:3000). .. Signals were developed using ECL solution (Thermo; 32106) and immunoreactive bands were visualized by LAS-4000.

Western Blot:

Article Title: Epstein-Barr viral product-containing exosomes promote fibrosis and nasopharyngeal carcinoma progression through activation of YAP1/FAPα signaling in fibroblasts.
Article Snippet: Treatment of Brefeldin A (BFA; BD Biosciences, 555,029) was used to increase the detectability of intracellular cytokines by Western blotting. .. The indicated antibodies against the following proteins were used for Western blotting: calnexin (Cell Signaling, #2679), phospho-YAP (Cell Signaling, #13008), YAP (Cell Signaling, #14074), CYR61 (Cell Signaling, #14479), CTGF (Cell Signaling, #86641), IGFBP3 (Cell Signaling, #25864), PDGFRα/β (Abcam, ab32570), α-SMA (Abcam, ab124964), phospho-mTOR (Cell Signaling, #2971), phospho-p70S6K1 (Cell Signaling, #9234.), phospho-MEK1/2 (Cell Signaling, #9121), MEK1/2 (Cell Signaling, #4694), phospho-p38 MAPK (Cell Signaling, #4511), p38 MAPK (Cell Signaling, #9212), phospho-ERK1/2 (Cell Signaling, #4370), and ERK1/2 (Cell Signaling, #4695), CD9 (Abcam, ab92726), GAPDH (Abcam, ab9484), active YAP1 (Abcam, ab205270), mTOR (Abcam, ab2732), p70S6K1 (Abcam, ab32529), HSP70 (System Biosciences, EXOAB-HSP70A-1), β-actin (Sigma, A5060), α-tubulin (Santa Cruz Biotechnology, sc32293), and EBV-encoded LMP1 monoclonal antibody (S12), purified from hybridoma culture supernatants. ..

Purification:

Transduction:

Article Title: PKC-eta promotes breast cancer metastasis by regulating the Hippo-YAP signaling pathway.
Article Snippet: .. The antibodies used in this study were against YAP (CST, #14074), MST1 (CST, #3682), pMST1 (CST, #3681), LATS1 (CST, #3477), 14-3-3 ζ/δ (CST, #7413), pLATS1 (CST, #8654), pYAP (Ser127) (CST, Signal Transduction and Targeted Therapy (2026) 11:58 #13008), pYAP (Ser109) (CST, #53749), pYAP (Ser397) (CST, #13619), pTAZ (Ser89) (CST, #75275), TAZ (CST, #83669), PTEN (CST, #9559) Lamin B1 (CST, #13435), AKT (pan) (CST, #4691), AXL (CST, #8661), Pan-TEAD (CST, #13295), CYR61 (CST, 14479), IGFBP3 (CST, #25864), HA-Tag (CST, #3724), FLAG (CST, #14793), pAKT (Ser473) (CST, #4060), and Anti-rabbit IgG (CST, # 7074). .. Epithelial–Mesenchymal Transition (EMT) Antibody Sampler Kit, CST, #9782 [(Vimentin (D21H3), Snail (C15D3), Slug (C19G7), ZEB1 (D80D3), N-cadherin (D4R1H), ZO-1 (D7D12), E-cadherin (24E10)]. pYAP (Ser128) (Thermo Fisher Scientific, # PA5-117264), PKCη (Santa Cruz, #sc215), PKCε (BD Biosciences, #610085), PKCδ (BD Biosciences, #610398), PKCα (BD Biosciences, #610108), PKCζ (Abcam #ab59364), PKCη (Abcam #ab179524), EpCAM (Abcam, ab223582), and β-actin (ICN Biomedicals Inc., 691001) were used.

Image Search Results

Quantification of cytokine array analysis performed with supernatants from murine macrophages treated with rGST or rGST-Otlip or salivary gland lysates generated from unfed or fed ticks. The bar graphs depicting the differential levels (as a fold change) of murine cytokines/chemokines in supernatants collected from murine macrophages treated with rGST-Otlip relative to the levels noted upon treatment with rGST (A) or treated with fed tick salivary gland lysate relative to the levels noted upon treatment with unfed tick salivary gland lysates (B) . The cytokine/chemokines with the highest level in the fold change is at the top of the bar graph while the lowest level in the fold change is at the bottom of the bar graph. A fold change value of 1 indicates no change in the expression levels. The graphs were generated using Power-BI software. Protein names are indicated on the Y-axis. IGFBP3 protein is boxed in both arrays.

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: Quantification of cytokine array analysis performed with supernatants from murine macrophages treated with rGST or rGST-Otlip or salivary gland lysates generated from unfed or fed ticks. The bar graphs depicting the differential levels (as a fold change) of murine cytokines/chemokines in supernatants collected from murine macrophages treated with rGST-Otlip relative to the levels noted upon treatment with rGST (A) or treated with fed tick salivary gland lysate relative to the levels noted upon treatment with unfed tick salivary gland lysates (B) . The cytokine/chemokines with the highest level in the fold change is at the top of the bar graph while the lowest level in the fold change is at the bottom of the bar graph. A fold change value of 1 indicates no change in the expression levels. The graphs were generated using Power-BI software. Protein names are indicated on the Y-axis. IGFBP3 protein is boxed in both arrays.

Article Snippet: After gel electrophoresis, blots were blocked with 5% milk buffer and probed with IGFBP3 rabbit polyclonal antibody (cat. no. A16052, ABclonal, USA) or Caspase-3 p12 Rabbit monoclonal antibody (cat. no. A19664, ABclonal, USA) or Bcl2 Rabbit polyclonal antibody (cat. vo.

Techniques: Generated, Expressing, Software

Treatment of murine macrophages with rGST-Otlip or salivary gland lysates modulates cytokine gene expression. qRT-PCR analysis showing transcript levels of fas -ligand (A) , lix (B) , igfbp-3 (C) , il -3 (D) and gcsf (E) in murine macrophage cells upon treatment with rGST or rGST-Otlip. qRT-PCR analysis showing transcript levels of fas -ligand (F) , il -17 (G) , igfbp-3 (H) , vcam -1 (I) and teck (J) in murine macrophage cells upon treatment with salivary gland lysates generated from unfed or fed O. turicata americanus ticks. Open circles indicate data from samples generated upon treatment with rGST or salivary gland lysates from unfed ticks and closed circles represent data from samples generated upon treatment with rGST-Otlip or salivary gland lysates from fed ticks. The transcript levels of cytokines were normalized to mouse actin transcript levels. Statistical analysis was performed using Mann-Whitney U test and P value is shown.

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: Treatment of murine macrophages with rGST-Otlip or salivary gland lysates modulates cytokine gene expression. qRT-PCR analysis showing transcript levels of fas -ligand (A) , lix (B) , igfbp-3 (C) , il -3 (D) and gcsf (E) in murine macrophage cells upon treatment with rGST or rGST-Otlip. qRT-PCR analysis showing transcript levels of fas -ligand (F) , il -17 (G) , igfbp-3 (H) , vcam -1 (I) and teck (J) in murine macrophage cells upon treatment with salivary gland lysates generated from unfed or fed O. turicata americanus ticks. Open circles indicate data from samples generated upon treatment with rGST or salivary gland lysates from unfed ticks and closed circles represent data from samples generated upon treatment with rGST-Otlip or salivary gland lysates from fed ticks. The transcript levels of cytokines were normalized to mouse actin transcript levels. Statistical analysis was performed using Mann-Whitney U test and P value is shown.

Techniques: Gene Expression, Quantitative RT-PCR, Generated, MANN-WHITNEY

rGST-Otlip treatment causes significant upregulation of apoptotic gene expression in murine raw macrophages. qRT-PCR analysis showing expression of igfbp-3R (A) , bax (B) , caspase-9 (C) , caspase-3 (D) , and bcl- 2 (E) in murine macrophage cells upon treatment with rGST or rGST-Otlip. The mRNA levels of cytokines were normalized to mouse actin mRNA levels. Statistical analysis was performed using Mann-Whitney U test and P value is shown. Open circles indicate data from samples generated upon treatment with rGST and closed circles represent data from samples generated upon treatment with rGST-Otlip. (F) Immunoblotting analysis showing levels of IGFBP3 and Caspase-3 in murine macrophage cells upon treatment with rGST or rGST-Otlip. Beta-actin levels serve as loading control in the immunoblotting analysis. Protein sizes are indicated as kilodaltons (kDa).

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: rGST-Otlip treatment causes significant upregulation of apoptotic gene expression in murine raw macrophages. qRT-PCR analysis showing expression of igfbp-3R (A) , bax (B) , caspase-9 (C) , caspase-3 (D) , and bcl- 2 (E) in murine macrophage cells upon treatment with rGST or rGST-Otlip. The mRNA levels of cytokines were normalized to mouse actin mRNA levels. Statistical analysis was performed using Mann-Whitney U test and P value is shown. Open circles indicate data from samples generated upon treatment with rGST and closed circles represent data from samples generated upon treatment with rGST-Otlip. (F) Immunoblotting analysis showing levels of IGFBP3 and Caspase-3 in murine macrophage cells upon treatment with rGST or rGST-Otlip. Beta-actin levels serve as loading control in the immunoblotting analysis. Protein sizes are indicated as kilodaltons (kDa).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, MANN-WHITNEY, Generated, Western Blot, Control

Treatment of murine macrophages with O. turicata americanus salivary gland lysate induces apoptotic gene expression. qRT-PCR analysis showing expression of igfbp-3R (A) , bax (B) , caspase-9 (C) , caspase-3 (D) , and bcl-2 (E) transcripts in murine macrophages upon treatment with salivary gland lysates generated from O. turicata americanus unfed or fed nymphal ticks. The transcript levels of cytokines were normalized to mouse beta-actin transcript levels. Statistical analysis was performed using Mann-Whitney U test and P values are shown. Open circles indicate data from samples generated upon treatment with salivary gland lysates from unfed ticks and closed circles represent data from samples generated upon treatment with salivary gland lysates from fed ticks. (F) Immunoblotting analysis showing expression of IGFBP3 and Caspase-3 protein in murine macrophages upon treatment with O. turicata americanus unfed or fed salivary gland lysates. Beta-actin levels serve as loading control. Protein sizes are indicated as kilodaltons (kDa).

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: Treatment of murine macrophages with O. turicata americanus salivary gland lysate induces apoptotic gene expression. qRT-PCR analysis showing expression of igfbp-3R (A) , bax (B) , caspase-9 (C) , caspase-3 (D) , and bcl-2 (E) transcripts in murine macrophages upon treatment with salivary gland lysates generated from O. turicata americanus unfed or fed nymphal ticks. The transcript levels of cytokines were normalized to mouse beta-actin transcript levels. Statistical analysis was performed using Mann-Whitney U test and P values are shown. Open circles indicate data from samples generated upon treatment with salivary gland lysates from unfed ticks and closed circles represent data from samples generated upon treatment with salivary gland lysates from fed ticks. (F) Immunoblotting analysis showing expression of IGFBP3 and Caspase-3 protein in murine macrophages upon treatment with O. turicata americanus unfed or fed salivary gland lysates. Beta-actin levels serve as loading control. Protein sizes are indicated as kilodaltons (kDa).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Generated, MANN-WHITNEY, Western Blot, Control

Otlip treatment modulates apoptotic gene and protein expression in human keratinocytes: qRT-PCR analysis showing expression of igfbp-3 (A) , caspase-3 (B) and bcl-2 (C) in HaCaT cells upon treatment with rGST or rGST-Otlip. The mRNA levels of cytokines were normalized to human beta-actin mRNA levels. Open circles indicate data from samples generated upon treatment with rGST and closed circles represent data from samples generated upon treatment with rGST-Otlip. Statistical analysis was performed using Mann-Whitney U test and P value is shown. (D) Immunoblotting analysis showing expression of IGFBP3, Caspase -3 and BCL-2 proteins in HaCaT cells upon treatment with rGST or rGST-Otlip. Beta-actin levels serve as loading controls. Protein sizes are indicated as kilodaltons (kDa).

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: Otlip treatment modulates apoptotic gene and protein expression in human keratinocytes: qRT-PCR analysis showing expression of igfbp-3 (A) , caspase-3 (B) and bcl-2 (C) in HaCaT cells upon treatment with rGST or rGST-Otlip. The mRNA levels of cytokines were normalized to human beta-actin mRNA levels. Open circles indicate data from samples generated upon treatment with rGST and closed circles represent data from samples generated upon treatment with rGST-Otlip. Statistical analysis was performed using Mann-Whitney U test and P value is shown. (D) Immunoblotting analysis showing expression of IGFBP3, Caspase -3 and BCL-2 proteins in HaCaT cells upon treatment with rGST or rGST-Otlip. Beta-actin levels serve as loading controls. Protein sizes are indicated as kilodaltons (kDa).

Techniques: Expressing, Quantitative RT-PCR, Generated, MANN-WHITNEY, Western Blot

Knockdown of igfbp3 expression affects rGST-Otlip-induced apoptosis in murine macrophages. qRT-PCR analysis showing expression of igfbp3 (A) , caspase-3 (B) and bcl-2 (C) in igfbp3/scrambled -siRNA-rGST-Otlip-treated macrophages. The mRNA levels of igfbp3 (A) , caspase-3 (B) and bcl-2 (C) were normalized to mouse beta-actin mRNA levels. Open circles indicate data from scrambled siRNA-treated group and closed circles represent data from igfbp3 -siRNA-treated group. Statistical analysis was performed using Mann-Whitney U test and P values are shown. (D) Immunoblotting analysis showing expression of IGFBP3 and Caspase -3 proteins in igfbp3/scrambled -siRNA-rGST-Otlip-treated macrophages. Mouse beta-actin levels serve as loading controls. Protein sizes are indicated as kilodaltons (kDa). (E) Schematic representation showing the role of O. turicata americanus lipocalin in the modulation of apoptosis via IGFBP3 signaling in mammalian cells. While feeding on a mammalian host, ticks release salivary cocktail containing histamine-binding lipocalin. The secreted tick lipocalin stimulates the production of IGFBP-3 (Insulin-like growth factor-binding protein-3) and its receptor IGFBP-3R in both murine macrophages and human keratinocytes. The secreted IGFBP-3 could then bind to IGFBP-3R to stimulate apoptotic cascade. Otlip induces mammalian Caspase 9 (initiator caspase), Caspase 3 (executioner caspase) and repress BCL-2 (anti-apoptotic) to induce apoptosis in macrophages and keratinocytes. Induction in apoptosis of immune cells like macrophages and resident cells like keratinocytes would enable ticks to successfully complete their blood feeding.

Journal: Frontiers in Immunology

Article Title: Tick lipocalin triggers mammalian IGFBP-3-mediated apoptosis in macrophages and keratinocytes

doi: 10.3389/fimmu.2026.1768484

Figure Lengend Snippet: Knockdown of igfbp3 expression affects rGST-Otlip-induced apoptosis in murine macrophages. qRT-PCR analysis showing expression of igfbp3 (A) , caspase-3 (B) and bcl-2 (C) in igfbp3/scrambled -siRNA-rGST-Otlip-treated macrophages. The mRNA levels of igfbp3 (A) , caspase-3 (B) and bcl-2 (C) were normalized to mouse beta-actin mRNA levels. Open circles indicate data from scrambled siRNA-treated group and closed circles represent data from igfbp3 -siRNA-treated group. Statistical analysis was performed using Mann-Whitney U test and P values are shown. (D) Immunoblotting analysis showing expression of IGFBP3 and Caspase -3 proteins in igfbp3/scrambled -siRNA-rGST-Otlip-treated macrophages. Mouse beta-actin levels serve as loading controls. Protein sizes are indicated as kilodaltons (kDa). (E) Schematic representation showing the role of O. turicata americanus lipocalin in the modulation of apoptosis via IGFBP3 signaling in mammalian cells. While feeding on a mammalian host, ticks release salivary cocktail containing histamine-binding lipocalin. The secreted tick lipocalin stimulates the production of IGFBP-3 (Insulin-like growth factor-binding protein-3) and its receptor IGFBP-3R in both murine macrophages and human keratinocytes. The secreted IGFBP-3 could then bind to IGFBP-3R to stimulate apoptotic cascade. Otlip induces mammalian Caspase 9 (initiator caspase), Caspase 3 (executioner caspase) and repress BCL-2 (anti-apoptotic) to induce apoptosis in macrophages and keratinocytes. Induction in apoptosis of immune cells like macrophages and resident cells like keratinocytes would enable ticks to successfully complete their blood feeding.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, MANN-WHITNEY, Western Blot, Binding Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: PKC-eta promotes breast cancer metastasis by regulating the Hippo–YAP signaling pathway

doi: 10.1038/s41392-026-02572-0

Figure Lengend Snippet: AKT phosphorylation of the upstream kinase cascade of the Hippo pathway is negatively regulated by PKCη. a Western blot analysis showing the activation of members of the upstream kinase cascade of the Hippo pathway, which is regulated by AKT phosphorylation, in PKCη KO TNBC cells. PKCη promotes YAP and PTEN expression, leading to dephosphorylation and inactivation of AKT. b An AKT inhibitor (MK-2206) effectively inhibits the upstream Hippo pathway phospho-cascade regulated by AKT in PKCη KO TNBC cells. c YAP-specific siRNA downregulates PTEN expression in TNBC cells in conjunction with AKT activation. d Relative mRNA expression of PTEN and PRKCH in control and MDA-MB-231 PKCη KO cells, as determined by RT‒qPCR. Knockout of PKCη in MDA-MB-231 cells resulted in a marked reduction in PTEN transcript levels compared with those in control cells. e Impact of PKCη expression on YAP transcriptional targets in TNBC cells. f Relative mRNA expression of canonical YAP target genes ( AXL, CYR61, IGFBP3 , and TEAD ) was measured via RT‒qPCR in MDA-MB-231 PKCη KO and control cells. PKCη knockout results in a significant reduction in the expression of these downstream effectors. g Schematic illustration of how YAP activity regulates PTEN expression and modulates the AKT pathway through this feedback mechanism (in the presence or absence of PKCη). Statistical significance was determined via two-way ANOVA, where * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Article Snippet: The antibodies used in this study were against YAP (CST, #14074), MST1 (CST, #3682), pMST1 (CST, #3681), LATS1 (CST, #3477), 14-3-3 ζ/δ (CST, #7413), pLATS1 (CST, #8654), pYAP (Ser127) (CST, #13008), pYAP (Ser109) (CST, #53749), pYAP (Ser397) (CST, #13619), pTAZ (Ser89) (CST, #75275), TAZ (CST, #83669), PTEN (CST, #9559) Lamin B1 (CST, #13435), AKT (pan) (CST, #4691), AXL (CST, #8661), Pan-TEAD (CST, #13295), CYR61 (CST, 14479), IGFBP3 (CST, #25864), HA-Tag (CST, #3724), FLAG (CST, #14793), pAKT (Ser473) (CST, #4060), and Anti-rabbit IgG (CST, # 7074).

Techniques: Phospho-proteomics, Western Blot, Activation Assay, Expressing, De-Phosphorylation Assay, Control, Knock-Out, Activity Assay

Review

igfbp3 (Cell Signaling Technology Inc)

Structured Review

Images

1) Product Images from "PKC-eta promotes breast cancer metastasis by regulating the Hippo–YAP signaling pathway"

Related Articles

Blocking Assay:

Incubation:

Western Blot:

Purification:

Transduction:

Similar Products

Image Search Results